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Published on: February 1, 2014
Real-time multiplex PCR for detecting Shiga toxin 2-producing Escherichia coli O104:H4 in human stools
Wenlan Zhang1, Martina Bielaszewska, Andreas Bauwens
1Institute for Hygiene and the National Consulting Laboratory for Hemolytic Uremic Syndrome, University of Münster, Münster, Germany.
Journal of Clinical Microbiology
|February 17, 2012
Abstract:
A real-time multiplex PCR targeting stx(2), wzy(O104), and fliC(H4) of enterohemorrhagic Escherichia coli (EHEC) O104:H4 correctly determined the presence or absence of these genes in 253 EHEC isolates and enrichment cultures of stool samples from 132 patients. It is a rapid, sensitive, and specific tool for detecting EHEC O104:H4 in human stools.

