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Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

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Published on: March 20, 2018

Image formation in the scanning transmission electron microscope using object-conjugate detectors.

C Dwyer¹, S Lazar, L Y Chang

¹Monash Centre for Electron Microscopy, Monash University, Victoria 3800, Australia. christian.dwyer@monash.edu

Acta Crystallographica. Section A, Foundations of Crystallography

|February 18, 2012

Summary

This study analyzes image formation in scanning transmission electron microscopy (STEM). Geometric optics accurately approximates real-space STEM imaging, simplifying interpretation and implementation.

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Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

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Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Published on: March 12, 2017

Area of Science:

Electron microscopy
Optics and imaging

Background:

Image formation in scanning transmission electron microscopy (STEM) is complex.
Existing techniques include scanning confocal electron microscopy (SCEM) and real-space scanning transmission electron microscopy (R-STEM).

Purpose of the Study:

To theoretically analyze image formation in STEM with detectors conjugate to the specimen.
To evaluate the validity of geometric optics versus wave optics for R-STEM and SCEM.
To provide conditions for accurate geometric optics interpretation.

Main Methods:

Theoretical analysis using wave and geometric optics.
Wigner distribution analysis to assess geometric optics validity.
Comparison of theoretical models with experimental data for R-STEM.

Main Results:

Geometric optics provides an accurate approximation for R-STEM, especially with large detectors.
This geometric approach offers advantages for R-STEM interpretation and numerical implementation.
SCEM geometric optics is valid under specific conditions: negligible higher-order aberrations and sufficiently large detectors.

Conclusions:

Geometric optics is a powerful tool for understanding R-STEM image formation.
The findings simplify the analysis and application of R-STEM.
Conditions for valid geometric optics in SCEM are clarified.