Related Experiment Video
Updated: May 24, 2026

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants
Published on: May 1, 2013
Modulating intestinal immune responses by lipoteichoic acid-deficient Lactobacillus acidophilus
Mohammad W Khan1, Mojgan Zadeh, Praveen Bere
1Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA.
Aim:
To investigate the mechanism(s) by which the intestinal commensal microbe Lactobacillus acidophilus can affect host immunity, we studied the role of a component of the cell wall, lipoteichoic acid, in colitis.
Materials & Methods:
Colitis was induced by the intraperitoneal injection of pathogenic CD4(+)CD25(-)CD45RB(hi) T cells into Rag1(-/-) mice. The parental strain, NCK56, or the lipoteichoic acid-deficient strain, NCK2025, was then administered orally. Fluorescent microscopy was employed to examine resulting cell populations and their cytokine production in the colon.
Results:
NCK2025 enhanced IL-10 production by dendritic cells and macrophages. Increased numbers of regulatory dendritic cells coincided with the induction of activated FoxP3(+) Tregs.
Conclusion:
These results suggest that the oral administration of the genetically modified strain NCK2025 may be an effective immunotherapeutic approach that reprograms the immune response in colonic inflammatory conditions.
More Related Videos
06:57Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
Published on: September 18, 2016
07:34Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice
Published on: December 16, 2021
Related Concept Videos
Probiotics
Functions of the Gut Microbiota
Microbiota of the Stomach and Small Intestine
Microbiota of the Large Intestine
Renewal of Intestinal Stem Cells