Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

High-Performance Liquid Chromatography: Introduction

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:

High-Performance Liquid Chromatography: Instrumentation

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.

Gas Chromatography: Types of Columns and Stationary Phases

Gas Chromatography: Types of Columns and Stationary Phases

Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

Types Of Column Chromatography

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Uncertainty-driven model-based search methods for method development in liquid chromatography.

Journal of chromatography. A·2026

Same author

Automated micropillar array design with Bayesian optimization and computational fluid dynamics.

Journal of chromatography. A·2026

Same author

Computational study of the mass transfer effects in metal-organic framework columns for liquid chromatography.

Journal of chromatography. A·2026

Same author

Revisiting the definition and measurement of total accessible and hold-up volumes in liquid chromatography: Current understanding and advances.

Journal of chromatography. A·2026

Same author

SERS Substrate Fabrication via Rapid Triboelectrification-Driven Self-Assembly of Close-Packed Colloidal Monolayers.

Small methods·2026

Same author

Kinetic plot method for the optimal column and instrument design for high speed chiral SFC applications.

Journal of chromatography. A·2026

Search research articles

Related Experiment Video

Updated: May 24, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

Capillary liquid chromatography separations using non-porous pillar array columns.

Wim De Malsche¹, Selm De Bruyne, Jeff Op De Beek

¹Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium. wdemalsc@vub.ac.be

Journal of Chromatography. A

|February 21, 2012

Summary

This summary is machine-generated.

A novel non-porous pillar array column (NP-PAC) coupled with capillary LC offers high performance separations. This microfluidics system achieves over 100,000 theoretical plates, demonstrating its potential for small molecule analysis.

More Related Videos

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

Related Experiment Videos

Last Updated: May 24, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

Area of Science:

Analytical Chemistry
Separation Science
Microfluidics

Background:

Traditional liquid chromatography (LC) faces challenges with efficiency and throughput.
Microfluidic devices offer potential for miniaturized and high-performance separations.

Purpose of the Study:

To evaluate the performance of a non-porous pillar array column (NP-PAC) integrated with a commercial capillary LC system.
To demonstrate the practical applicability of the NP-PAC for various small molecule analyses.

Main Methods:

Coupling a non-porous pillar array column (NP-PAC) to a capillary LC instrument.
Utilizing large pillar dimensions (11 μm) and a multi-channel design (20 tracks) for increased channel length (1.4m) and volume (28μl).
Performing van Deemter analysis, gradient, and isocratic separations with various sample types (parabens, phenones, sulfonamides, steroids, BSA digest).

Main Results:

Achieved a minimal plate height of 13 μm, yielding approximately 100,000 theoretical plates in 30 minutes under non-retained conditions.
Demonstrated stable operation for 3 months with ~500 injections.
Obtained good to reasonable peak capacities (n(p)=100-140 in 50-70 min) and peak symmetries in both gradient and isocratic modes.
Quantification figures of merit in the ppm range were achieved.

Conclusions:

The NP-PAC system integrated with capillary LC provides high separation efficiency and robustness.
This microfluidics-based approach shows significant promise for high-throughput and sensitive small molecule analysis.
The developed system overcomes interfacing dispersion issues common in microfluidic LC systems.