Related Experiment Video
Updated: May 24, 2026
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Published on: April 12, 2019
Antiproliferative effect of α-mangostin on canine osteosarcoma cells
Aungkana Krajarng1, Sirinun Nilwarankoon, Sunit Suksamrarn
1Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand.
Abstract:
Osteosarcoma is the most frequently diagnosed primary bone tumor in dog. Since chemotherapeutics are quite limited due to high cost and severe toxicity, therefore, the ultimate goal is to discover cost-effective therapeutics with less toxicity. We have studied the effect of α-mangostin, a xanthone derivative isolated from pericarp of mangosteen (Garcinia mangostana Linn.) in canine osteosarcoma, D-17 cells. The results showed that α-mangostin induced antiproliferation with IC(50) at 15 μg/ml. Hoechst 33342 nuclear staining and nucleosomal DNA-gel electrophoresis revealed that α-mangostin could induce nuclear condensation and fragmentation, typically seen in apoptosis. Cell cycle analysis demonstrated that α-mangostin induced sub-G1 peak. In addition, α-mangostin also induced membrane flipping of the phosphatidylserine and the loss of mitochondrial membrane potential in D-17 cells. In conclusion, α-mangostin, induced apoptotic cell death against canine osteosarcoma D-17 cells, could be a potential candidate for preventive and therapeutic application for bone cancer treatment in dogs.
Insights
Alpha-mangostin, a compound from mangosteen, effectively inhibits canine osteosarcoma cell growth. This natural compound induces apoptosis, offering a potential cost-effective and less toxic treatment for canine bone cancer.
Area of Science:
- Veterinary Oncology
- Pharmacology
- Natural Product Chemistry
Background:
- Osteosarcoma is the most common primary bone tumor in dogs.
- Current chemotherapeutics for canine osteosarcoma are limited by high cost and severe toxicity.
- There is a need for cost-effective and less toxic therapeutic options.
Purpose of the Study:
- To investigate the anti-cancer effects of alpha-mangostin, a xanthone derivative from Garcinia mangostana Linn. (mangosteen).
- To evaluate the potential of alpha-mangostin as a therapeutic agent for canine osteosarcoma.
Main Methods:
- The study utilized the D-17 cell line, a canine osteosarcoma model.
- Assays included antiproliferation (IC50 determination), Hoechst 33342 nuclear staining, DNA-gel electrophoresis, cell cycle analysis, phosphatidylserine flipping assay, and mitochondrial membrane potential assessment.
Main Results:
- Alpha-mangostin demonstrated significant antiproliferative effects against D-17 cells with an IC50 of 15 μg/ml.
- Morphological and biochemical analyses indicated that alpha-mangostin induces apoptosis, characterized by nuclear condensation, fragmentation, and a sub-G1 cell cycle peak.
- The compound also induced phosphatidylserine externalization and loss of mitochondrial membrane potential, further supporting apoptotic cell death.
Conclusions:
- Alpha-mangostin effectively induces apoptotic cell death in canine osteosarcoma D-17 cells.
- Alpha-mangostin shows promise as a potential candidate for preventive and therapeutic applications in treating canine bone cancer.
- Further research into alpha-mangostin could lead to novel, cost-effective, and safer treatments for canine osteosarcoma.
