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Updated: May 24, 2026

Identification of Mycobacterium Species by DNA Microarray Chip Method
Published on: June 24, 2025
Dynamic and complex Mycobacterium tuberculosis microevolution unrevealed by standard genotyping
Laura Pérez-Lago1, Marta Herranz, Emilio Bouza
1Servicio de Microbiología y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, C/ Dr Esquerdo, 46, 28007 Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
Abstract:
We performed an in-depth analysis of a large Mycobacterium tuberculosis cluster involving nine isolates with identical or highly similar RFLP types. The strain involved in this transmission cluster microevolved and accumulated up to five differential IS6110 transposition events, several of them not revealed by standard genotyping approaches. Some of these events probably generated extended deletions. In two cases of tuberculosis within the cluster, we observed the simultaneous presence of both the reference and microevolved variants. A longitudinal genotypical survey of M. tuberculosis in a real clinical evolutionary scenario can reveal dynamic and complex microevolution events.
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