Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

RPSA-OLFM4 axis governs neutrophil migration against bacterial infection and sepsis.

Nature communications·2026

Same author

Single-cell landscape of piglet lung response with <i>Actinobacillus pleuropneumoniae</i>.

Virulence·2026

Same author

The <i>iucC</i> gene is a major contributor to the virulence of hypervirulent <i>Klebsiella pneumoniae</i>.

Frontiers in cellular and infection microbiology·2026

Same author

Targeting fimbriae adhesin of porcine enterotoxigenic Escherichia coli by isoliquiritigenin.

International journal of medical microbiology : IJMM·2026

Same author

Design of B-Cell Multi-Epitope Subunit Vaccines Against <i>Glaesserella parasuis</i> by Reverse Vaccinology: An In Silico and In Vivo Study.

Transboundary and emerging diseases·2026

Same author

Evaluation of the cross-immunogenicity and protective efficacy of the multi-epitope antigen Tzi from Glasserella parasuis in mouse model.

Vaccine·2025

Same journal

Synergistic Antibacterial Activity of Shikonin Compounds Combined with Conventional Antibiotics Against Methicillin-Resistant Staphylococcus Aureus (MRSA).

Microbial pathogenesis·2026

Same journal

Staphylococcus aureus strain heterogeneity modulates virulence traits associated to dual-species biofilms with Candida albicans.

Microbial pathogenesis·2026

Same journal

Host Immunosuppression and Antifungal Pressure Cooperatively Shape Fungal Adaptation and Pulmonary Microenvironment in Cryptococcosis.

Microbial pathogenesis·2026

Same journal

Dihalogenated indoles with antimicrobial activity against C. acnes and polymicrobial biofilms.

Microbial pathogenesis·2026

Same journal

Ethanol extract of Trichoderma harzianum spores exhibits antibacterial activity against Staphylococcus aureus coupled with free radical scavenging.

Microbial pathogenesis·2026

Same journal

Coumarin Derivatives Targeting Ergosterol and Sphingolipid Pathways to Inhibit Candida albicans: Molecular, Metabolomic, and Drosophila Toxicity Insights.

Microbial pathogenesis·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 24, 2026

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Published on: August 5, 2016

Deep sequencing-based expression transcriptional profiling changes during Brucella infection.

Qianhong Liu¹, Wenyu Han, Changjiang Sun

¹College of Animal Science and Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun 130062, PR China.

Microbial Pathogenesis

|February 21, 2012

Summary

This summary is machine-generated.

This study reveals how Brucella melitensis infection alters host macrophage gene expression, particularly affecting apoptosis and cellular pathways within 24 hours. Understanding these host responses is key to developing new strategies against brucellosis.

More Related Videos

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays

Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays

Published on: July 22, 2022

Related Experiment Videos

Last Updated: May 24, 2026

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Published on: August 5, 2016

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays

Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays

Published on: July 22, 2022

Area of Science:

Microbiology
Immunology
Genomics

Background:

Brucellosis is a global zoonotic disease impacting animal and human health.
Host macrophage interaction with Brucella is crucial for infection establishment.
Understanding host cell responses is vital for preventing Brucella infections.

Purpose of the Study:

To investigate the kinetic transcriptional profile of gene expression in macrophages infected with Brucella melitensis strain 16M.
To identify host cell pathways and genes significantly altered during the early stages of infection.

Main Methods:

Deep sequencing technology was employed to analyze gene expression.
Total RNA was extracted from macrophages at 0, 4, and 24 hours post-infection.
Gene ontology and pathway enrichment analyses were performed.

Main Results:

Significant changes in gene expression were observed in the endoplasmic reticulum, lysosomes, and pathways related to apoptosis and programmed cell death within 24 hours.
Key pathways affected include apoptosis, NOD-like receptor signaling, Fc gamma R-mediated phagocytosis, lysosome, p53 signaling, and protein processing in the endoplasmic reticulum.
B-cell receptor and toll-like receptor signaling pathways showed significant alterations between 4 and 24 hours post-infection.

Conclusions:

The study provides insights into host macrophage responses to Brucella melitensis infection.
Identified transcriptional changes highlight critical cellular processes manipulated by the bacteria.
Findings can inform the development of novel therapeutic strategies against brucellosis.