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Pyrosequencing for Microbial Identification and Characterization
Published on: August 22, 2013
Application of pyrosequencing for Salmonella enterica rapid identification
Xiujuan Li1, Fang Yang, Weili Gao
1Microbiology Laboratory, Shijiazhuang Center for Disease Control and Prevention, 3 Likang Street, Shijiazhuang, Hebei, China.
Journal of Microbiological Methods
|February 21, 2012
Abstract:
Application of pyrosequencing of six Salmonella-specific genes as a rapid Salmonella identification method was tested. Primers for hns, hisJ and hilA had non-specific reactions with non-Salmonella strains. Primers for invA, iroB and fimY had specific PCR products and pyrosequences of Salmonella, suggesting that they were suitable for Salmonella rapid identification.
