Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nanostructured interfaces integrated with unsupervised intelligence to mitigate global polycrisis complexities.

Advances in colloid and interface science·2026

Same author

Characterization and evaluation of the ability of graphene quantum dots to affect α-synuclein aggregation in synucleinopathy models.

Science and technology of advanced materials·2026

Same author

Corrigendum to "Microwave-assisted assembly of Ag<sub>2</sub>O-ZnO composite nanocones for electrochemical detection of 4-Nitrophenol and assessment of their photocatalytic activity towards degradation of 4-nitrophenol and methylene blue dye" [J Hazard Mater 416 (2021) 125771].

Journal of hazardous materials·2026

Same author

Transcending Metal Organic Framework chemistry: Zeolitic Imidazolate Framework derivatives as tunable catalysts for solar, electrocatalysis, and biocatalytic remediation.

Advances in colloid and interface science·2026

Same author

Selenium-Integrated MOFs and Their Derived Materials for Advanced Design, Functionality, and Applications.

ACS applied materials & interfaces·2026

Same author

Biomimetic Nanoparticles for Targeted and Efficient Cancer Therapy: Progress, Challenges and Perspectives.

International journal of nanomedicine·2026

Search research articles

Related Experiment Video

Updated: Feb 16, 2026

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Published on: September 7, 2018

Nano-structured arrays for multiplex analyses and Lab-on-a-Chip applications.

Chandra K Dixit¹, Ajeet Kaushik

¹National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland. Chandra.dixit2@mail.dcu.ie

Biochemical and Biophysical Research Communications

|February 21, 2012

Summary

Researchers developed a novel nano-structured array (NA) biochip using gold and microfluidics for sensitive protein detection. This platform offers a stable and efficient solution for multiplex analysis and biosensing applications.

More Related Videos

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Related Experiment Videos

Last Updated: Feb 16, 2026

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Published on: September 7, 2018

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Area of Science:

Materials Science and Nanotechnology
Biotechnology and Biosensing
Analytical Chemistry

Background:

Existing biosensing platforms often lack efficiency and cost-effectiveness for multiplex analysis.
Nano-structured arrays (NAs) offer high surface area for enhanced molecular interactions.
Microfluidics enables precise sample handling and integration of detection systems.

Purpose of the Study:

To fabricate a cost-effective nano-structured array (NA) gold platform using nanospheres lithography (NSL).
To integrate the NA platform with a microfluidics system for multiplex analysis.
To evaluate the platform's performance for biosensing applications using a model recognition system.

Main Methods:

Fabrication of hexagonally close-packed gold (Au) nano-structured arrays (NAs) using polystyrene beads (PS) as a mask via NSL.
Integration of the NA platform with a customized microfluidics system.
Demonstration of chip functionality using horseradish peroxidase (HRP) and anti-HRP antibody, including enzyme-linked immunosorbent assay (ELISA).

Main Results:

The fabricated protein biochip achieved a detection limit of 100 pg/mL for HRP.
The antibody chips exhibited a shelf-life of 50 days when stored at 4°C with no significant loss of activity.
The NA-based protein biochip demonstrated efficient performance in a microfluidics system.

Conclusions:

The NSL-fabricated NA gold platform integrated with microfluidics provides an efficient and cost-effective solution for biosensing.
The developed protein biochip shows high sensitivity and stability, suitable for diagnostics.
This platform holds significant potential for advancing multiplex analysis and Lab-on-a-Chip applications in diagnostics and biosensing technology.