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Related Experiment Video

Updated: May 24, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes T&#252;6028
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From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Published on: January 13, 2017

Combinatorial biosynthesis of polyketides--a perspective.

Fong T Wong1, Chaitan Khosla

  • 1Department of Chemical Engineering, Stanford University, Stanford, CA 94305, United States.

Current Opinion in Chemical Biology
|February 21, 2012
PubMed
Summary
This summary is machine-generated.

Combinatorial biosynthesis engineering of polyketide synthases shows promise for creating novel bioactive molecules. Overcoming scientific and technological hurdles is key to advancing this field for drug discovery.

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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
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Last Updated: May 24, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes T&#252;6028
09:08

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Published on: January 13, 2017

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Area of Science:

  • Biotechnology
  • Synthetic Biology
  • Natural Product Synthesis

Background:

  • Polyketide synthases (PKS) are crucial enzymes for producing diverse natural products.
  • Engineering PKS through combinatorial biosynthesis offers a route to 'unnatural' natural products with tailored bioactivities.
  • Despite two decades of progress, combinatorial biosynthesis remains an emerging field with significant potential.

Purpose of the Study:

  • To review the scientific and technological challenges impeding combinatorial biosynthesis.
  • To propose strategies for accelerating the adoption of PKS engineering for bioactive molecule preparation.
  • To foster the development of tools and algorithms for PKS-based drug discovery.

Main Methods:

  • Enzyme-centric perspective on polyketide synthase engineering.
  • Discussion of current limitations and future directions in combinatorial biosynthesis.
  • Proposal of a periodic challenge program, inspired by protein structure prediction.

Main Results:

  • Identified key scientific and technological bottlenecks in combinatorial biosynthesis.
  • Highlighted the need for standardized methods and validation in PKS engineering.
  • Proposed a collaborative challenge framework to drive innovation.

Conclusions:

  • Accelerating combinatorial biosynthesis requires addressing specific scientific and technological challenges.
  • A structured, community-driven approach (e.g., periodic challenges) can foster rapid advancement.
  • This field holds significant promise for the discovery of novel bioactive small molecules.