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Updated: May 24, 2026
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The Stroke Preclinical Assessment Network Multi-Laboratory Model of Thromboembolic Stroke with Thrombolysis: TE-MCAo
Published on: December 19, 2025
Thrombotic stroke in the anesthetized monkey (Macaca mulatta): characterization by MRI--a pilot study
Maxime Gauberti1, Pauline Obiang, Pierre Guedin
1INSERM U919, Serine Proteases and Pathophysiology of the Neurovascular Unit, GIP Cyceron, Université de Caen-Basse Normandie, Caen, France.
Cerebrovascular Diseases (Basel, Switzerland)
|February 21, 2012
Summary
Researchers developed a new embolic stroke model in nonhuman primates. This clinically relevant primate model aids in testing new diagnostic and therapeutic strategies for cerebrovascular disease.
Area of Science:
- Neuroscience
- Primate Models
- Cerebrovascular Disease Research
Background:
- Lack of suitable large nonhuman primate stroke models impedes development of diagnostic and therapeutic approaches for cerebrovascular disease.
- Need for a clinically relevant embolic stroke model in primates for advanced research.
Purpose of the Study:
- To develop a novel, clinically relevant model of embolic stroke in anesthetized nonhuman primates.
- To incorporate readily available clinical imaging techniques and enable drug delivery/reperfusion strategies.
Main Methods:
- Thrombin injection into the middle cerebral artery (MCA) of 12 anesthetized rhesus macaques.
- Sequential MRI studies including angiography, FLAIR, PWI, DWI, and gadolinium-enhanced T1W imaging at 3T.
- Monitoring of physiological and biochemical parameters throughout the investigation.
Main Results:
- Standardized procedure successfully induced transient MCA occlusion in all animals.
- Spontaneous reperfusion observed between 2 hours and 7 days post-ictus in all subjects.
- Eighty percent exhibited diffusion/perfusion mismatch; ischemic lesions spared MCA territories; some showed hemorrhagic transformation.
Conclusions:
- A pre-clinically relevant model of embolic stroke in anesthetized nonhuman primates was successfully developed.
- This model provides a valuable platform for advancing stroke research and therapeutic development.
