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Related Experiment Video

Updated: May 24, 2026

Anterior Segment Organ Culture Platform for Tracking Open Globe Injuries and Therapeutic Performance

Anterior Segment Organ Culture Platform for Tracking Open Globe Injuries and Therapeutic Performance

Published on: August 25, 2021

Microbial contamination in open globe injury.

S Bhala¹, S Narang, S Sood

¹Department of Ophthalmology and Microbiology, Government Medical College Hospital, Chandigarh, India. soniya.bhala@gmail.com

Nepalese Journal of Ophthalmology : a Biannual Peer-Reviewed Academic Journal of the Nepal Ophthalmic Society : NEPJOPH

|February 21, 2012

Summary

Microbial contamination occurs in 26% of open globe injuries (OGI), with Aspergillus being the most common contaminant. Early contamination significantly correlates with endophthalmitis development, highlighting the need for close follow-up.

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Area of Science:

Ophthalmology
Infectious Diseases
Microbiology

Background:

Endophthalmitis is a severe complication of ocular trauma.
Identifying microbial contaminants is crucial for effective empirical antibiotic therapy.

Purpose of the Study:

To determine the incidence of microbial contamination in open globe injuries (OGI).
To identify the spectrum of microorganisms involved in OGI.

Main Methods:

A prospective study of 50 consecutive OGI cases over two years.
Intraoperative sampling of conjunctival sac and anterior chamber.
Cultures of abscised tissue, foreign bodies, and vitreous taps when indicated.

Main Results:

Microbial cultures were positive in 26% of cases.
Aspergillus species were the most frequent contaminant (45.6%), followed by Alternaria and Curvularia.
A significant association was found between initial contamination and the development of endophthalmitis (p<0.05).

Conclusions:

Initial contamination affects 26% of OGI cases, with fungi like Aspergillus being predominant.
A strong correlation exists between initial contamination and subsequent endophthalmitis.
Culture-negative cases showed a trend towards better visual outcomes; close follow-up of contaminated OGI cases is recommended.