Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Focusing of Light in the Eye01:16

Focusing of Light in the Eye

Light rays enter the eye through the cornea, a transparent dome-shaped tissue that is the eye's outermost layer. The cornea bends or refracts, light rays traveling to the pupil. The shape of the cornea determines how much of the light is bent and whether the image will be focused correctly on the retina at the back of the eye. Once the light has passed through both refraction layers, it converges into a single focal point onto a small area. This is where photoreceptors start transforming...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Observing a focused weak shock wave perform a Hilbert transform.

Physical review. E·2026
Same author

4polar3D single molecule imaging of 3D orientation in dense actin networks using ratiometric polarization splitting.

Nature communications·2026
Same author

Farewell and greetings from the outgoing and incoming editors: editorial.

Optics letters·2026
Same author

Spiral adaptive Fresnel lens: generalizations, improvements, and augmented-reality/virtual-reality simulations.

Journal of the Optical Society of America. A, Optics, image science, and vision·2025
Same author

Spiral adaptive Fresnel lens: wave optics.

Journal of the Optical Society of America. A, Optics, image science, and vision·2025
Same author

Geometric phases and coherent states for commensurate bichromatic polarization and astigmatic cavities.

Nanophotonics (Berlin, Germany)·2025
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: May 24, 2026

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
07:14

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

Published on: April 11, 2025

Imaging with complex ray-optical refractive-index interfaces between complex object and image distances.

Johannes Courtial1, Blair C Kirkpatrick, Miguel A Alonso

  • 1School of Physics and Astronomy, University of Glasgow, Glasgow, United Kingdom.

Optics Letters
|February 21, 2012
PubMed
Summary
This summary is machine-generated.

This study defines objects and images at complex positions for optical components. An example optical component demonstrates imaging between real and complex object/image locations, advancing complex refractive index research.

More Related Videos

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
10:01

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

Related Experiment Videos

Last Updated: May 24, 2026

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
07:14

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

Published on: April 11, 2025

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
10:01

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

Area of Science:

  • Optics
  • Materials Science

Background:

  • Recent research models optical windows as interfaces between materials.
  • These interfaces feature a complex ray-optical refractive index ratio.

Purpose of the Study:

  • To define objects and images at complex positions in optical systems.
  • To demonstrate an optical component capable of imaging between real and complex object/image planes.

Main Methods:

  • Theoretical definition of objects and images at complex coordinates.
  • Development and analysis of a specific optical component design.

Main Results:

  • Successful definition of objects and images in complex space.
  • Demonstration of an optical component performing real-to-complex and complex-to-real imaging.

Conclusions:

  • The framework allows for analysis of optical systems with complex parameters.
  • The demonstrated component validates the theoretical concepts for complex imaging.