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Europium-labeled activity-based probe through click chemistry: absolute serine protease quantification using (153)Eu
Xiaowen Yan1, Yacui Luo, Zhubao Zhang
1Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, China.
Abstract:
Click and analyze: the titled probe was synthesized by conjugating a sulfonyl fluoride and azido unit using click chemistry to give SF-Eu, which can react specifically with serine (Ser) in the active site of serine protease (SP). Combination of the method with (153)Eu-isotope dilution ICP/MS enables absolute protein quantification of active SPs in biological samples using only one (153)Eu(NO(3))(3) isotopic standard.
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