Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

9.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Layer-by-Layer Assembly of Conductive MOFs/PEDOT:PSS Hybrid Films with Superior Areal Capacitance for Flexible Transparent Supercapacitors.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

The impact of dietary patterns on the risk of asthma in children.

Allergy and asthma proceedings·2026
Same author

Symptom phenotypes and their associations with fear of cancer recurrence and social support among breast cancer patients: a latent profile analysis.

Scientific reports·2026
Same author

Selective interaction of SMCHD1 with chromatin is governed by LRIF1 and SMCHD1 ATPase activity.

Nature communications·2026
Same author

Machine Learning Accelerated Non-Adiabatic Molecular Dynamics Elucidates Local Polarization Effects on Non-radiative Recombination in Halide Perovskites.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Traditional Chinese medicine reverses immune checkpoint resistance through lactate metabolic reprogramming in hepatocellular carcinoma: Mechanisms and therapeutic implications.

Journal of ethnopharmacology·2026
Same journal

Optimized tRNA structure-seq reveals robust tRNA secondary structures in <i>S. cerevisiae</i> under mild stress conditions.

RNA (New York, N.Y.)·2026
Same journal

SERIPH: A Two-Step Extraction Protocol for Selective Enrichment of Semi-Extractable RNAs.

RNA (New York, N.Y.)·2026
Same journal

Reduced Sensitivity to RNA Structural Differences Distinguishes Eukaryotic Pus4 from Bacterial TruB.

RNA (New York, N.Y.)·2026
Same journal

Puf3 contributes to changes in mRNA solubility, translation elongation dynamics at rare arginine codons and loss of protein homeostasis in cells lacking Not4.

RNA (New York, N.Y.)·2026
Same journal

RBM38 Regulates HORMAD1 Splicing to Enhances MEK Inhibitor Sensitivity in Breast Cancer.

RNA (New York, N.Y.)·2026
Same journal

EF-P Inhibits Ribosomal α-Hydroxy Acid Incorporation: Strategic tRNA Body Selection for Co-incorporating α-Hydroxy Acids and Nonproteinogenic Amino Acids into Depsipeptides.

RNA (New York, N.Y.)·2026
See all related articles

Related Experiment Video

Updated: May 1, 2026

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

16.5K

A differential sequencing-based analysis of the C. elegans noncoding transcriptome.

Tengfei Xiao1, Yunfei Wang, Huaxia Luo

  • 1Laboratory of Bioinformatics and Noncoding RNA, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

RNA (New York, N.Y.)
|February 21, 2012
PubMed
Summary
This summary is machine-generated.

Researchers identified 473 novel intermediate-size noncoding RNAs (is-ncRNAs) in C. elegans, revealing new RNA classes and potential functions in gene regulation and development.

More Related Videos

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology
08:19

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

Published on: May 28, 2020

7.6K
Hand Dissection of Caenorhabditis elegans Intestines
05:41

Hand Dissection of Caenorhabditis elegans Intestines

Published on: September 13, 2022

5.3K

Related Experiment Videos

Last Updated: May 1, 2026

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

16.5K
High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology
08:19

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

Published on: May 28, 2020

7.6K
Hand Dissection of Caenorhabditis elegans Intestines
05:41

Hand Dissection of Caenorhabditis elegans Intestines

Published on: September 13, 2022

5.3K

Area of Science:

  • Molecular Biology
  • Genomics
  • RNA Biology

Background:

  • Noncoding RNAs are crucial in eukaryotic biology.
  • Nonpolyadenylated and intermediate-size noncoding RNAs (is-ncRNAs) in Caenorhabditis elegans remain understudied.
  • Previous transcriptome mapping efforts have not fully characterized these RNA types.

Purpose of the Study:

  • To comprehensively identify and characterize novel is-ncRNAs in C. elegans.
  • To investigate the potential functions and evolutionary relationships of these novel RNAs.

Main Methods:

  • Combined enzymatic approaches with full-length RNA sequencing (RNA-Seq) for is-ncRNA analysis.
  • Sequence and secondary structure analysis for RNA classification.
  • In vitro binding experiments and structure-based clustering.

Main Results:

  • Identified 473 novel is-ncRNAs, many associated with transcription factor binding sites and developmental expression.
  • Classified over 200 is-ncRNAs into known classes and discovered two new groups (33 is-ncRNAs).
  • Found potential homologs of human BC200 RNA and Y RNAs, and identified modified snoRNAs.

Conclusions:

  • This study significantly expands the catalog of C. elegans is-ncRNAs.
  • Identified novel RNA classes and potential functional roles in gene regulation and development.
  • Suggests a broader diversity of functional RNAs derived from processed and modified primary transcripts.