Molecular Identification and Sequencing of Mannose Binding Protein (MBP) Gene of Acanthamoeba palestinensis

Area of Science:

• Microbiology
• Ophthalmology
• Molecular Biology

Background:

• Acanthamoeba keratitis is a severe eye infection caused by pathogenic Acanthamoeba species.
• Acanthamoeba palestinensis is a significant causative agent of amoebic keratitis, particularly in Iran.
• Mannose Binding Protein (MBP) is identified as a primary virulence factor in Acanthamoeba pathogenesis.

Purpose of the Study:

• To characterize the Mannose Binding Protein (MBP) gene in pathogenic Acanthamoeba isolates, specifically Acanthamoeba palestinensis.
• To understand the genetic basis of MBP's role in Acanthamoeba keratitis.

Main Methods:

• Acanthamoeba palestinensis isolates were cultured using standard microbiological techniques.
• DNA was extracted using the phenol-chloroform method.
• Polymerase Chain Reaction (PCR) was employed with specific primers to amplify the MBP gene fragment, followed by purification, sequencing, and gene bank deposition.

Main Results:

• A 900 bp PCR product was successfully amplified.
• Sequence analysis revealed a complete gene sequence of 943 nucleotides.
• Homology analysis indicated 81% similarity to existing MBP gene sequences in the data bank, with the fragment deposited under accession number EU678895.

Conclusions:

• The Mannose Binding Protein (MBP) plays a critical role in the Acanthamoeba pathogenesis cascade.
• Characterization of the MBP gene provides a foundation for developing novel therapeutic strategies.
• This research may contribute to the development of vaccines for individuals at high risk of Acanthamoeba keratitis.