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Related Concept Videos

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

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Related Experiment Video

Updated: May 24, 2026

Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration
10:09

Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration

Published on: February 28, 2013

Genomic analysis of wig-1 pathways.

Yalda Sedaghat1, Curt Mazur, Mahyar Sabripour

  • 1Department of Drug Discovery, Isis Pharmaceuticals Inc, Carlsbad, California, United States of America.

Plos One
|February 21, 2012
PubMed
Summary

Wig-1 (wild-type p53-induced gene 1) suppression using antisense oligonucleotides effectively reduced wig-1 levels in mouse liver and brain. This targeted gene regulation impacted pathways relevant to neurodegenerative diseases and cancer.

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Area of Science:

  • Molecular Biology
  • Neuroscience
  • Genetics

Background:

  • Wig-1 (wild-type p53-induced gene 1) is a p53-regulated transcription factor crucial for RNA and protein stabilization.
  • Wig-1 interacts with hnRNP A2/B1, RNA Helicase A, and dsRNAs, and stabilizes p53 mRNA.
  • Dysregulation of p53 and its targets, like wig-1, are implicated in neurodegenerative diseases.

Purpose of the Study:

  • To investigate the role of wig-1 as a downstream p53 target.
  • To characterize the effects of wig-1 downregulation on gene expression in mouse liver and brain.
  • To assess the therapeutic potential of targeting wig-1 in disease models.

Main Methods:

  • Development and application of antisense oligonucleotides (ASOs) targeting mouse wig-1 mRNA.
  • Intraperitoneal and striatal administration of wig-1 ASOs in FVB and BACHD mice.
  • Gene expression analysis using expression microarrays.

Main Results:

  • Wig-1 ASOs effectively reduced wig-1 mRNA and protein levels in mouse liver and brain.
  • Wig-1 suppression lowered mutant huntingtin protein in BACHD mouse brains without affecting normal huntingtin or p53 levels.
  • Genome-wide expression analysis revealed significant upregulation and downregulation of various genes following wig-1 reduction.

Conclusions:

  • Antisense oligonucleotides provide a viable method for reducing wig-1 levels in vivo.
  • Wig-1 downregulation induces specific gene expression changes relevant to neurological disorders and cancer pathways.
  • Targeting wig-1 represents a potential therapeutic strategy for diseases involving p53 pathway dysregulation.