Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

piRNA - Piwi-interacting RNAs

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

High mobility group box 1 (HMGB1) levels in the placenta and in serum in preeclampsia.

American journal of reproductive immunology (New York, N.Y. : 1989)·2011

Same author

Destabilization of coxsackievirus b3 genome integrated with enhanced green fluorescent protein gene.

Intervirology·2011

Same author

[Clinicopathological features of primary splenic histiocytic sarcoma: a case report and literature review].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2011

Same author

[Comparison of treatment with micro endoscopic discectomy and posterior lumbar interbody fusion using single and double B-Twin expandable spinal spacer].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2011

Same author

Virtual transplantation in designing a facial prosthesis for extensive maxillofacial defects that cross the facial midline using computer-assisted technology.

The International journal of prosthodontics·2011

Same author

Total synthesis of phorboxazole A via de novo oxazole formation: convergent total synthesis.

Journal of the American Chemical Society·2010

Search research articles

Related Experiment Video

Updated: May 24, 2026

Processing the Loblolly Pine PtGen2 cDNA Microarray

Processing the Loblolly Pine PtGen2 cDNA Microarray

Published on: March 20, 2009

Microarray identification of conserved microRNAs in Pinellia pedatisecta.

Bo Wang¹, Miao Dong, Wenduo Chen

¹College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China.

|February 22, 2012

Summary

This summary is machine-generated.

Researchers identified 99 microRNAs (miRNAs) in Pinellia pedatisecta, an important medicinal plant. This study is the first to report on miRNAs in this species, paving the way for future research on their developmental roles.

More Related Videos

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Related Experiment Videos

Last Updated: May 24, 2026

Processing the Loblolly Pine PtGen2 cDNA Microarray

Processing the Loblolly Pine PtGen2 cDNA Microarray

Published on: March 20, 2009

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Area of Science:

Plant molecular biology
Genomics
Medicinal botany

Background:

MicroRNAs (miRNAs) are crucial regulators of plant development.
Pinellia pedatisecta, a significant aroid medicinal plant, lacks reported miRNA studies.
This species is unique for its pedate leaf blades, large tubers, and inflorescences.

Purpose of the Study:

To identify and characterize microRNAs (miRNAs) in Pinellia pedatisecta.
To establish a foundation for understanding miRNA functions in this species.
To investigate the expression patterns of selected miRNAs in different tissues.

Main Methods:

In situ synthesized custom miRNA microarray for detection.
Reverse transcription polymerase chain reaction (RT-PCR) for validation.
Quantitative RT-PCR (qRT-PCR) for expression analysis.

Main Results:

A total of 99 miRNAs from 22 miRNA families were identified in P. pedatisecta.
Microarray results were validated using RT-PCR for 14 selected miRNAs.
qRT-PCR revealed differential expression levels of seven miRNAs across various tissues.

Conclusions:

This research presents the first comprehensive report of miRNAs in Pinellia pedatisecta.
The identified miRNAs and their expression data provide a valuable resource for future studies.
Further investigation into the roles of these miRNAs in P. pedatisecta development is warranted.