Related Experiment Video
Updated: May 24, 2026

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
Published on: March 24, 2012
Characterization and functional analysis of methionine sulfoxide reductase A gene family in tomato
Changbo Dai1, Myeong-Hyeon Wang
1Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University, Chuncheon, Gangwon-do, 200-701, South Korea.
Abstract:
In this study, four methionine sulfoxide reductase A (MSRA) genes, SlMSRA2-5, were identified in Micro-Tom (Solanum lycopersicum cv.'Micro-Tom') based on the tomato database. Exon/intron distribution assays indicated that the gene sequence of SlMSRA2, SlMSRA3, and SlMSRA4 contained two exons and one intron in the tomato genome, while the coding sequence of SlMSRA5 was interrupted by three introns. Southern blot analysis revealed that the members of the MSRA family existed as low copies in the tomato genome. Although SlMSRA2 and E4 gene (one of ethylene-inducible gene) share a similarity of 93.4% in terms of their amino acid sequence, organ-specific expression revealed that gene expression were remarkably different based on Northern blot analysis. In addition, SlMSRA3 and SlMSRA4 transcripts were abundant in leaves while expression of SlMSRA5 was weak in most of organs examined. The expression pattern of SlMSRAs induced in response to hormones and stresses suggested that the tomato MSRA genes might be involved in resistance against pathogens and various abiotic stresses. In addition, kinetics parameters suggested that recombinant SlMSRA4 protein exhibited a highest affinity for dabsyl-labeled methionine sulfoxide and its catalytic efficiency (K (cat) /K (m) ) was threefold and 6.5-fold higher than that of SlMSRA2 and SlMSRA3. However, the activity of purified SlMSRA5 was not detected in the DTT-dependent reducing system.
More Related Videos
09:05Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
Published on: March 11, 2020
10:40Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
Published on: December 22, 2017
Related Concept Videos
Sulfur Assimilation
Microtubule Associated Motor Proteins
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Gene Families
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Cis-regulatory Sequences