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Updated: Jul 22, 2026
Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
Published on: July 19, 2019
Gastric cancer cell lines AGS before and after CD40 signal activating
Rui Li1, Xue-Qin Pang, Wei-Chang Chen
1Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Key Laboratory of Medicine and Clinical Immunology of Jiangsu Province, Suzhou, China.
Abstract:
The aim of this study was to investigate the molecular mechanisms underlying the antitumour effects of CD40L through analysing the change of genes expression profile in AGS using Affymetrix Gene Chip. Human gastric carcinoma AGS cells were first incubated with 2 μg/ml sCD40L or equal volume of medium (control) in F12 medium. RNA was isolated from AGS and were reverse transcribed, labeled with digoxigenin-11-dUTP, and then hybridized with Clontech Atlas mouse cDNA expression arrays for comparison. Performing clustering analysis, we found that 7 detected genes were down-regulated and 38 were upregulated as the sCD40L acted on AGS. To further verify the results of gene chip screening, Gene Database was searched, finding that the most significantly up-regulated genes were Gadd45a, c-Jun and Bcl-2, and the most significantly down-regulated genes were Cyclin D1, CDC6, TNFR10B, c-IAP2 and ORC5L. Based upon these findings, the signalling pathways that possibly mediate CD40-induced apoptosis are proposed.
Insights
Soluble CD40 Ligand (sCD40L) triggers gene expression changes in human gastric carcinoma cells, revealing molecular pathways involved in its antitumor effects. This study identifies key upregulated and downregulated genes, offering insights into CD40-mediated apoptosis.
Area of Science:
- Molecular Biology
- Cancer Research
- Immunology
Background:
- Gastric carcinoma poses a significant health challenge.
- Understanding the molecular basis of antitumor therapies is crucial for developing effective treatments.
- CD40 Ligand (CD40L) is a key immune-modulating protein with potential anticancer applications.
Purpose of the Study:
- To investigate the molecular mechanisms of soluble CD40 Ligand (sCD40L) in human gastric carcinoma (AGS) cells.
- To analyze changes in gene expression profiles induced by sCD40L treatment.
- To identify potential signaling pathways mediating CD40-induced apoptosis in gastric cancer.
Main Methods:
- Human gastric carcinoma AGS cells were treated with sCD40L.
- Gene expression profiling was performed using Affymetrix Gene Chip technology.
- Clustering analysis and database searches were employed to identify differentially expressed genes.
Main Results:
- sCD40L treatment led to significant alterations in gene expression in AGS cells.
- 38 genes were found to be upregulated, including Gadd45a, c-Jun, and Bcl-2.
- 7 genes were downregulated, including Cyclin D1, CDC6, TNFR10B, c-IAP2, and ORC5L.
Conclusions:
- The study elucidates molecular mechanisms underlying the antitumor effects of sCD40L.
- Identified gene expression changes provide insights into CD40-mediated apoptosis pathways in gastric cancer.
- Findings contribute to understanding CD40L's role in cancer immunotherapy.
