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Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Analysis of ethanol in fermentation samples by a robust nanocomposite-based microbial biosensor.
Jana Sefčovičová1, Jaroslav Filip, Vladimír Mastihuba
1Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Bratislava, SK, Slovakia.
Biotechnology Letters
|February 22, 2012
Summary
A novel microbial biosensor using Gluconobacter oxydans and carbon nanotubes offers sensitive ethanol detection. This robust device demonstrates high stability and rapid analysis for fermentation samples.
Area of Science:
- Biotechnology
- Biosensor Technology
- Nanomaterials
Background:
- Ethanol detection is crucial in fermentation monitoring.
- Existing methods may lack sensitivity or speed.
- Development of rapid and sensitive biosensors is needed.
Purpose of the Study:
- To construct a robust microbial biosensor for ethanol detection.
- To enhance sensitivity and stability using a bionanocomposite.
- To evaluate the biosensor's performance in flow injection analysis.
Main Methods:
- Direct mixing of Gluconobacter oxydans and carbon nanotubes.
- Utilizing ferricyanide as a mediator for ethanol oxidation.
- Integration into a flow injection analysis (FIA) system.
Main Results:
- Achieved high sensitivity of (74 ± 2.7) μA mM(-1) cm(-2) and a low detection limit of 5 μM.
- Demonstrated a linear detection range from 10 μM to 1 mM.
- Exhibited a short response time (67 h(-1) sample throughput) and high operational stability (1.7% response decrease over 43 h).
Conclusions:
- The developed microbial biosensor is robust, sensitive, and stable.
- The bionanocomposite approach enhances ethanol detection capabilities.
- The biosensor is suitable for analyzing ethanol in fermentation samples, showing good agreement with HPLC.
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