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Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
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Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

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Updated: May 24, 2026

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
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Split-transgene expression in wheat.

Mario Gils1, Myroslava Rubtsova, Katja Kempe

  • 1Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) Gatersleben, Gatersleben, Germany. gils@ipk-gatersleben.de

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2012
PubMed
Summary

Researchers developed a split-gene technique for controlling traits in plants. This method creates herbicide-resistant, pollen-sterile wheat using intein-mediated protein trans-splicing for precise gene expression control.

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Area of Science:

  • Plant biotechnology
  • Molecular biology
  • Genetics

Background:

  • Controlling plant traits and gene flow is crucial for agriculture.
  • Split-gene approaches offer precise control over transgene expression.

Purpose of the Study:

  • To describe a methodology for producing herbicide-resistant and pollen-sterile wheat plants.
  • To present intein-mediated protein trans-splicing as a tool for split-gene applications in plants.

Main Methods:

  • Intein-mediated assembly of inactive precursor protein fragments (protein trans-splicing).
  • Design of intein-containing vectors for split-transgene expression.
  • Transient plant assays for system functionality analysis.
  • Wheat plant transformation using a split selection marker.

Main Results:

  • Successful production of herbicide-resistant and pollen-sterile wheat plants.
  • Demonstration of split-gene functionality in wheat using transient assays and transformation.

Conclusions:

  • Intein-mediated split-gene technology is a feasible approach for trait and gene flow control in wheat.
  • The described methodology provides a guideline for applying split-gene strategies in monocotyledonous crops.