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Related Experiment Video

Updated: May 24, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Published on: May 20, 2013

UPLC-MS-based metabolite analysis in tomato.

Ilana Rogachev1, Asaph Aharoni

  • 1Department of Plant Sciences, The Weizmann Institute of Science, Rehovot, Israel. ilana.rogachev@weizmann.ac.il

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2012
PubMed
Summary
This summary is machine-generated.

Ultraperformance Liquid Chromatography (UPLC) coupled with quadrupole time-of-flight (qTOF) mass spectrometry enables extensive plant metabolomics. This method efficiently profiles semipolar metabolites in tomato, advancing our understanding of plant biochemistry.

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Last Updated: May 24, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
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Published on: May 20, 2013

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Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

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Area of Science:

  • Plant Science
  • Analytical Chemistry
  • Biochemistry

Background:

  • Hyphenated technologies combining chromatography and mass spectrometry are advancing metabolomics.
  • Ultraperformance Liquid Chromatography (UPLC) and high-sensitivity mass spectrometry offer enhanced performance for biological studies.

Purpose of the Study:

  • To describe the application of a UPLC-qTOF system for profiling semipolar metabolites in tomato.
  • To provide optimized methods for extraction, instrument parameters, and data treatment for plant metabolomics.

Main Methods:

  • Utilized Ultraperformance Liquid Chromatography (UPLC) with small particle size columns for high resolution and separation.
  • Employed a quadrupole time-of-flight (qTOF) mass spectrometer with high resolution, mass accuracy, and fast spectral acquisition.
  • Developed and applied optimized extraction, instrument, and data processing protocols.

Main Results:

  • Demonstrated the suitability of the UPLC-qTOF system for extensive profiling of hundreds of plant metabolites.
  • Highlighted the advantages of UPLC, including superior resolution, separation, and short injection times.
  • Showcased the high resolution, mass accuracy, and rapid data acquisition capabilities of the qTOF mass spectrometer.

Conclusions:

  • The UPLC-qTOF system is highly effective for comprehensive metabolomic profiling in plants.
  • Optimized protocols facilitate the detailed analysis of semipolar metabolites in model organisms like tomato.
  • This approach significantly advances the field of plant metabolomics and biochemical discovery.