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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Competitive ELISA.

K Makarananda1, G E Neal

  • 1Department of Pharmacology, Mahidol University, Bangkok, Thailand.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2012
PubMed
Summary
This summary is machine-generated.

Enzyme-linked immunosorbent assay (ELISA) provides sensitive detection of compounds like aflatoxins. This method is valuable for monitoring environmental toxins in food and biological samples.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Environmental Science

Background:

  • Enzyme-linked immunosorbent assay (ELISA) is a widely used technique for specific and sensitive compound detection.
  • The availability of specific antibodies (monoclonal or polyclonal) is crucial for ELISA's success.
  • ELISA is applicable to various matrices, including foodstuffs and biological fluids.

Purpose of the Study:

  • To highlight the utility of ELISA in detecting environmental contaminants and toxins.
  • To emphasize the application of ELISA in monitoring carcinogenic mycotoxins, specifically aflatoxins.

Main Methods:

  • Utilizes specific antibodies (monoclonal or polyclonal) for target compound recognition.
  • Employs enzyme-linked detection for sensitive quantification.
  • Applicable to diverse sample types such as food and human body fluids.

Main Results:

  • Demonstrates high specificity and sensitivity in compound assays.
  • Effectively monitors environmental contamination.
  • Shows increasing application in tracking carcinogenic mycotoxins like aflatoxins.

Conclusions:

  • ELISA is a powerful tool for sensitive and specific analysis of various compounds.
  • The technique is instrumental in environmental monitoring and toxicological studies.
  • ELISA is particularly valuable for the detection of hazardous substances such as aflatoxins.