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An immuno-slot-blot assay for detection and quantitation of alkyldeoxyguanosines in DNA

B I Ludeke1

  • 1Department of Neuropathology, University Hospital, Zurich, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2012
PubMed

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Southern Blot02:57

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...