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Screening of λgt11 cDNA Libraries Using Monoclonal Antibodies

D F Webster1, W T Melvin, M D Burke

  • 1Department of Molecular and Cell Biology, University of Aberdeen, Scotland.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2012
PubMed

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Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...