Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MDA-MB-231 cell morphology influences chemotactic sensing of CXCL12 gradients in type 1 bovine collagen matrix.

PloS one·2026
Same author

Leveraging nanoparticle protein corona to advance plasma proteome profiling.

Nature communications·2026
Same author

Metabolic Trojan Horse: Multivalent Glucose Ligand Modified Near-Infrared-Absorbing Gold Nanorods for Targeted Photothermal Therapy.

ACS applied materials & interfaces·2026
Same author

Real-Time Subcellular Imaging of Plant Signaling Molecules and Bio-Coronas by Near-Infrared Nanosensors.

ACS nano·2026
Same author

33 Unresolved Questions in Nanoscience and Nanotechnology.

ACS nano·2025
Same author

Casimir self-assembly: A platform for measuring nanoscale surface interactions in liquids.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Dec 26, 2025

Synthesis and Characterization of Amphiphilic Gold Nanoparticles
10:09

Synthesis and Characterization of Amphiphilic Gold Nanoparticles

Published on: July 2, 2019

18.0K

Evidence for patchy lipid layers on gold nanoparticle surfaces.

Jie An Yang1, Catherine J Murphy

  • 1Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|February 23, 2012
PubMed
Summary

Researchers explored ligand distribution on gold nanoparticles (AuNPs) for biomedical applications. They found that larger AuNPs show lipid patching, while smaller ones do not, impacting their use as multifunctional vehicles.

More Related Videos

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
08:09

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates

Published on: May 9, 2014

11.3K
Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry
14:18

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

Published on: October 4, 2011

14.8K

Related Experiment Videos

Last Updated: Dec 26, 2025

Synthesis and Characterization of Amphiphilic Gold Nanoparticles
10:09

Synthesis and Characterization of Amphiphilic Gold Nanoparticles

Published on: July 2, 2019

18.0K
A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
08:09

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates

Published on: May 9, 2014

11.3K
Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry
14:18

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

Published on: October 4, 2011

14.8K

Area of Science:

  • Nanotechnology
  • Materials Science
  • Biomedical Engineering

Background:

  • Gold nanoparticles (AuNPs) are promising for targeted drug delivery, imaging, and therapy.
  • Controlling ligand distribution on AuNP surfaces is crucial for optimizing their multifunctionality.
  • Quantitative analysis of ligand spatial arrangement on small nanoparticles (<100 nm) remains challenging.

Purpose of the Study:

  • To investigate the spatial organization of different lipids on varying sizes of gold nanoparticles (20, 50, and 90 nm).
  • To explore the impact of surface chemistry and nanoparticle size on lipid domain formation.
  • To develop and apply a novel angle analysis method for quantifying the arrangement of secondary nanoparticles on larger AuNPs.

Main Methods:

  • Functionalization of 20, 50, and 90 nm gold nanoparticles with single and mixed lipid systems using two distinct surface chemistries.
  • Mass spectrometry to confirm the presence of lipids in mixed systems.
  • Electron microscopy to visualize lipid domain formation and size.
  • Antibody-antigen conjugation for assembling 12 nm AuNPs onto 90 nm AuNPs.
  • Development and application of a new angle analysis procedure for quantifying secondary nanoparticle display.

Main Results:

  • Mass spectrometry confirmed the presence of both lipids in mixed-lipid functionalized AuNPs.
  • Electron microscopy revealed distinct lipid domains on 50 and 90 nm AuNPs, with domain sizes approximately 1/4 to 1/2 the particle diameter.
  • No evidence of lipid patching was observed on 20 nm AuNPs.
  • The assembly of 12 nm AuNPs onto 90 nm AuNPs was controllable via surface chemistry and quantified using the new angle analysis method.

Conclusions:

  • Lipid domain formation on gold nanoparticles is size-dependent, with smaller nanoparticles (<50 nm) exhibiting more homogeneous ligand distribution.
  • Surface chemistry influences lipid organization and the subsequent assembly of secondary nanoparticles.
  • The developed angle analysis provides a quantitative method to assess nanoparticle arrangement, crucial for designing advanced nanocarriers.