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Related Concept Videos

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Super-resolution Fluorescence Microscopy

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Related Experiment Video

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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Long-distance fluorescence lifetime imaging using stimulated emission.

Thilo Dellwig1, Po-Yen Lin, Fu-Jen Kao

  • 1National Yang-Ming University, Institute of Biophotonics, Taipei 11221, Taiwan.

Journal of Biomedical Optics
|February 23, 2012
PubMed
Summary

This study introduces long-distance fluorescence lifetime imaging, a novel technique for analyzing fluorescent materials. The method successfully measured fluorescence lifetimes of Rhodamine 6G and indocyanine green, advancing imaging capabilities.

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Area of Science:

  • Optics and Photonics
  • Spectroscopy
  • Biomedical Imaging

Background:

  • Long-distance stimulated emission imaging offers novel sample characterization.
  • Fluorescence lifetime is a critical parameter for identifying and quantifying fluorophores.
  • Existing methods may have limitations in distance or resolution.

Purpose of the Study:

  • To extend long-distance stimulated emission imaging for fluorescence lifetime determination.
  • To develop a pump-probe setup for enhanced fluorescence lifetime measurements.
  • To demonstrate the capability of long-distance fluorescence lifetime imaging.

Main Methods:

  • Utilized a pump-probe setup integrated with long-distance stimulated emission imaging.
  • Measured fluorescence lifetimes of Rhodamine 6G in various solutions.
  • Acquired fluorescence lifetime images of indocyanine green.

Main Results:

  • Successfully implemented a pump-probe system for long-distance fluorescence lifetime imaging.
  • Determined fluorescence lifetimes of Rhodamine 6G, showing sensitivity to solution environments.
  • Obtained fluorescence lifetime images of indocyanine green, validating the technique's applicability.

Conclusions:

  • Long-distance fluorescence lifetime imaging is a viable and powerful technique.
  • The developed method enables non-invasive characterization of fluorescent samples at a distance.
  • This advancement has potential applications in various fields requiring remote sample analysis.