Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ordering molecular diversity in untargeted metabolomics via molecular community networking.

Cell reports methods·2026
Same author

Chronic alcohol consumption disrupts the gut microbial and metabolic landscapes.

Frontiers in microbiology·2026
Same author

Small extracellular vesicle signaling and mitochondrial transfer reprogram T helper cell function in human asthma.

Nature communications·2026
Same author

Loss of proximal tubule lactate dehydrogenase A exacerbates nephrotoxic acute kidney injury through metabolic dysregulation.

American journal of physiology. Renal physiology·2026
Same author

Local Interactions Between Innate Immune Signaling, Microbiota, and Bile Acids Drive the Development of Duodenal Adenomas.

Cellular and molecular gastroenterology and hepatology·2025
Same author

Multiomic Analysis of the Gut Microbiome and Serum Metabolome in Response to a Low-Carbohydrate, High-Protein Diet in Individuals With Spinal Cord Injury.

Topics in spinal cord injury rehabilitation·2025

Related Experiment Video

Updated: May 24, 2026

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

The electrophile responsive proteome: integrating proteomics and lipidomics with cellular function.

Ashlee N Higdon1, Aimee Landar, Stephen Barnes

  • 1Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

Antioxidants & Redox Signaling
|February 23, 2012
PubMed
Summary

Lipid peroxidation modifies proteins, creating electrophile-responsive proteomes (ERP). Understanding these ERPs is key to disease pathology and developing targeted therapies.

More Related Videos

Lipidomics and Transcriptomics in Neurological Diseases
09:58

Lipidomics and Transcriptomics in Neurological Diseases

Published on: March 18, 2022

Related Experiment Videos

Last Updated: May 24, 2026

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

Lipidomics and Transcriptomics in Neurological Diseases
09:58

Lipidomics and Transcriptomics in Neurological Diseases

Published on: March 18, 2022

Area of Science:

  • Lipidomics and Proteomics
  • Redox Signaling
  • Post-Translational Protein Modification

Background:

  • Lipid peroxidation converts unsaturated fatty acids into reactive lipid species (RLS) within the oxylipidome.
  • Electrophilic RLS modify proteins at nucleophilic amino acids, forming electrophile-responsive proteomes (ERP).
  • This process links lipidomics and proteomics, impacting cellular function.

Purpose of the Study:

  • To explore the role of lipid peroxidation in protein modification.
  • To define electrophile-responsive proteomes (ERPs) and their formation mechanisms.
  • To highlight the connection between lipidomics and disease pathology.

Main Methods:

  • Advanced analytical techniques in lipidomics.
  • Investigation of enzymatic and nonenzymatic lipid peroxidation pathways.
  • Characterization of reactive lipid species (RLS) and their protein targets.

Main Results:

  • Biological systems utilize specific pro-oxidant pathways to generate RLS.
  • ERPs are formed through the reaction of RLS with proteins.
  • The study of ERPs is central to lipidomics and redox signaling research.

Conclusions:

  • A unique oxylipidome can predict biological responses via specific ERP formation.
  • ERPs modulate antioxidant and anti-inflammatory pathways.
  • ERPs are implicated in Keap1/Nrf2 activation and cell death pathways, including mitochondrial interactions.