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The Spindle Assembly Checkpoint

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...

The Spindle Assembly Checkpoint

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...

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Related Experiment Video

Updated: May 24, 2026

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

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Published on: September 20, 2019

A cell-based assay for screening spindle checkpoint inhibitors.

Zhen Hua Wu¹, Long Yu Hu, Da Qian Xu

¹School of Life Sciences, Xiamen University, Xiamen, Fujian, P.R. China.

Assay and Drug Development Technologies

|February 23, 2012

Summary

A new cell-based assay screens for spindle checkpoint inhibitors, which could lead to novel cancer drugs by targeting aneuploidy in tumor cells. This method is sensitive, cost-effective, and suitable for large-scale screening.

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Last Updated: May 24, 2026

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Published on: September 20, 2019

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Area of Science:

Cell Biology
Molecular Biology
Cancer Research

Background:

The spindle checkpoint is crucial for accurate chromosome segregation in eukaryotes, preventing aneuploidy.
Dysfunctional spindle checkpoints can lead to aneuploidy and potentially drive tumor formation.
Identifying spindle checkpoint inhibitors is a promising avenue for cancer drug development.

Purpose of the Study:

To develop and validate a cell-based assay for screening potential spindle checkpoint inhibitors.
To leverage the mechanism of spindle checkpoint inhibitors to identify novel anti-cancer compounds.

Main Methods:

Developed a cell-based assay to screen for compounds that induce cells to exit mitotic arrest.
Assay is based on the principle that spindle checkpoint inhibitors reverse drug-induced mitotic arrest.
Validated the assay using a known spindle checkpoint inhibitor.

Main Results:

The developed assay is sensitive and cost-effective.
The assay is adaptable for large-scale screening of potential drug candidates.
Successfully validated the assay's efficacy.

Conclusions:

The cell-based assay is a viable tool for identifying spindle checkpoint inhibitors.
This screening method offers a promising approach for discovering new cancer therapeutics targeting aneuploidy.