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Updated: May 24, 2026

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Published on: March 31, 2016

Feeding dried chicory root to pigs decrease androstenone accumulation in fat by increasing hepatic 3β hydroxysteroid

Martin Krøyer Rasmussen¹, Carl Brunius, Galia Zamaratskaia

¹Department of Food Science, Aarhus University, Denmark. Martink.rasmussen@agrsci.dk

The Journal of Steroid Biochemistry and Molecular Biology

|February 23, 2012

Summary

This summary is machine-generated.

Chicory root supplementation in pig feed significantly reduced adipose androstenone levels by increasing 3β-hydroxysteroid dehydrogenase (3β-HSD) enzyme activity. This dietary change also increased indole concentrations without affecting testosterone or IGF-1 levels.

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Area of Science:

Animal Science
Nutritional Biochemistry
Agricultural Science

Background:

Boar taint, primarily caused by androstenone accumulation in adipose tissue, is a significant concern in pig production.
Dietary interventions are explored to mitigate boar taint compounds and improve meat quality.
Chicory root (Cichorium intybus) contains various bioactive compounds with potential metabolic effects.

Purpose of the Study:

To investigate the in vivo effects of dietary chicory root on testicular steroid concentrations and androstenone-metabolizing enzymes in entire male pigs.
To assess the impact of chicory root on skatole and indole concentrations in plasma and adipose tissue.
To evaluate the influence of chicory root on specific enzyme expressions (3β-HSD, SULT2A1, HSP70) in liver tissue.

Main Methods:

A 16-day feeding trial with male pigs, comparing a diet with 10% dried chicory root against a standard control diet.
Analysis of plasma for testosterone, estradiol, IGF-1, skatole, and indole concentrations.
Measurement of androstenone, skatole, and indole in adipose tissue.
Quantification of mRNA and protein expression for 3β-HSD, SULT2A1, and HSP70 in liver tissue.

Main Results:

Chicory root significantly reduced androstenone concentrations in adipose tissue (p<0.05).
Indole concentrations in adipose tissue were significantly higher in pigs fed chicory root (p<0.05).
Dietary chicory root increased liver mRNA and protein expression of 3β-HSD and decreased HSP70 expression (p<0.05), without altering testosterone or IGF-1 plasma levels.

Conclusions:

Dietary chicory root effectively reduces androstenone levels in pig adipose tissue.
The mechanism involves the induction of 3β-hydroxysteroid dehydrogenase (3β-HSD) activity by chicory root.
These metabolic changes are not associated with increased cellular stress, as indicated by HSP70 expression.