Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development of Resistant GvHD in a Patient Treated with Nivolumab for Hodgkins Lymphoma Relapse after Allogeneic Unrelated Transplantation.

Klinicka onkologie : casopis Ceske a Slovenske onkologicke spolecnosti·2019

Same author

Comparison of autologous hematopoietic cell transplantation performed in tandem and in disease relapse in multiple myeloma patients.

Neoplasma·2018

Same author

Biochemical responses induced in galls of three Cynipidae species in oak trees.

Bulletin of entomological research·2017

Same author

Risk factors for treatment failure after allogeneic transplantation of patients with CLL: a report from the European Society for Blood and Marrow Transplantation.

Bone marrow transplantation·2017

Same author

Long-term survival of patients with CLL after allogeneic transplantation: a report from the European Society for Blood and Marrow Transplantation.

Bone marrow transplantation·2016

Same author

Prognostic factors to predict outcome of reduced intensity allogeneic haematopoietic cell transplantation for chronic lymphocytic leukemia.

Neoplasma·2016

Search research articles

Related Experiment Video

Updated: May 24, 2026

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Published on: February 10, 2017

Proteomic analysis of the presynaptic active zone.

W Volknandt¹, M Karas

¹Neurochemistry, Institute for Cell Biology and Neuroscience, Goethe University, Max-von-Laue-Str. 13, 60438 Frankfurt am Main, Germany. Volknandt@bio.uni-frankfurt.de

Experimental Brain Research

|February 23, 2012

Summary

Synaptic vesicles and their associated proteins are crucial for neuronal communication. Recent studies reveal the proteome of the active zone, identifying proteins involved in synaptic structure and function, including amyloid precursor protein.

More Related Videos

Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction

Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction

Published on: April 27, 2011

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

Published on: September 3, 2014

Related Experiment Videos

Last Updated: May 24, 2026

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Published on: February 10, 2017

Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction

Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction

Published on: April 27, 2011

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

Published on: September 3, 2014

Area of Science:

Neuroscience
Cell Biology
Proteomics

Background:

Synaptic vesicles mediate neuronal communication via protein functions.
Advances in mass spectrometry have detailed the synaptic vesicle proteome.
The active zone proteome is being actively investigated.

Purpose of the Study:

To identify proteins in the presynaptic active zone.
To understand the role of these proteins in synaptic structure and function.
To explore the relevance of amyloid precursor protein in the active zone.

Main Methods:

Immunoisolation of docked synaptic vesicles.
Gel-based protein separation techniques.
Mass spectrometry and immunodetection.

Main Results:

Identified a diverse set of proteins in the active zone.
Included synaptic vesicle proteins, fusion machinery, and signaling molecules.
Detected adhesion molecules and proteins regulating presynaptic dynamics.
Found amyloid precursor protein in active zone membrane fractions.

Conclusions:

The active zone proteome is complex and dynamic.
Identified proteins likely modulate presynaptic structure and function.
Amyloid precursor protein may interact with active zone partners.