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Related Concept Videos

Detailed Structure and Function of Lymph Nodes01:23

Detailed Structure and Function of Lymph Nodes

Lymph nodes are bean-shaped structures that cluster along the lymphatic vessels in the inguinal, axillary, and cervical regions. Each node is divided into compartments by a capsule that extends trabeculae inward.
From a histological perspective, lymph nodes can be split into two main areas: the superficial cortex and the deep medulla. The outer cortex is populated by dendritic cells, macrophages, and B lymphocytes, which are densely packed into follicles. When these B-lymphocytes are presented...

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Expression pattern changes and function of RANKL during mouse lymph node microarchitecture development.

Machiko Sugiyama1, Gaku Nakato, Toshi Jinnohara

  • 1Department of Supramolecular Biology, International Graduated School of Arts and Science Yokohama city university, Yokohama, Kanagawa 230-0045, Japan.

International Immunology
|February 23, 2012
PubMed
Summary
This summary is machine-generated.

Receptor activator of nuclear factor kappa-B ligand (RANKL) is critical for lymph node (LN) development. Blocking RANKL function in fetal mice completely inhibited LN development and perturbed B-cell follicle formation.

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Area of Science:

  • Immunology
  • Developmental Biology
  • Anatomy

Background:

  • Receptor activator of nuclear factor kappa-B ligand (RANKL) plays a role in immune system development.
  • Its expression pattern shifts during the development of fetal peripheral lymphoid organs, specifically in lymph node (LN) anlagen.

Purpose of the Study:

  • To investigate the functional role of RANKL during the development of mouse fetal peripheral lymphoid organs.
  • To understand the impact of RANKL expression changes on lymph node development.

Main Methods:

  • Examined RANKL expression during fetal lymphoid organ development in mice.
  • Blocked RANKL function using anti-RANKL antibodies administered to pregnant mice and directly into the amniotic space.
  • Observed effects on lymph node anlagen development, B-cell follicle formation, and high endothelial venule differentiation postnatally.

Main Results:

  • A shift in RANKL expression was observed during the transition from lymphoid tissue inducer (LTi) to lymphoid tissue organizer (LTo) cells in LN anlagen.
  • Complete blockade of RANKL function during a critical fetal period (13.5-16.5 dpc) completely inhibited LN anlagen development.
  • Direct injection of anti-RANKL antibodies into the amniotic space perturbed B-cell follicle formation and high endothelial venule differentiation.

Conclusions:

  • RANKL expression on LTi cells during early LN development is essential for LN microarchitecture formation.
  • RANKL signaling is a critical regulator of lymph node organogenesis.