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Updated: May 24, 2026
A Quantitative Detection Method for MicroRNAs in the Kidney of an Ischemic Kidney Injury Mouse Model
Published on: September 11, 2020
Validation of the isolation and quantification of kidney enriched miRNAs for use as biomarkers
Jack W Sharkey1, Daniel J Antoine, B Kevin Park
1MRC Centre for Drug Safety Science, Department of Molecular & Clinical Pharmacology, Institute of Translational Medicine, University of Liverpool, United Kingdom.
Context:
MiRNAs have been reported to represent sensitive and translational biomarkers of organ injury.
Objective:
To validate the methodologies for the isolation and quantification of a miRNAs from none-invasive biofluids.
Methods:
Commercially available miRNA isolation kits and qPCR was utilised for determination of analyte sensitivity, stability, recovery, and precision.
Results:
qPCR was highly precise and sensitive for endogenous miRNA quantification (miR-194, LLQ; 0.1 pM). Intra and inter-assay variation remained low (<12%). Variable recovery (54-89%) was controlled for by internal synthetic standards (C. elegans Lin-4).
Conclusions:
Translational endogenous miRNAs reflective of renal injury represent stable analytes with a large dynamic range that can be quantified easily in most laboratories.
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