Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In-plane dielectric constant and conductivity of confined water.

Nature·2025
Same author

The Surface-Topography Challenge: A Multi-Laboratory Benchmark Study to Advance the Characterization of Topography.

Tribology letters·2025
Same author

The tubular cavity of tobacco mosaic virus shields mechanical stress and regulates disassembly.

Acta biomaterialia·2025
Same author

Evaluation of the degradation of the graphene-polypropylene composites of masks in harsh working conditions.

Materials today. Chemistry·2022
Same author

Charge-polarized interfacial superlattices in marginally twisted hexagonal boron nitride.

Nature communications·2021
Same author

Atomic Force Microscopy of Viruses.

Advances in experimental medicine and biology·2019
Same journal

Unsupervised deep image prior for sparse-view and limited-angle electron tomography.

Ultramicroscopy·2026
Same journal

Determination of the structure of the tertiary phase in the alloy Al<sub>10</sub>Mo<sub>10</sub>Nb<sub>10</sub>Ta<sub>10</sub>Ti<sub>30</sub>Zr<sub>30</sub> using convergent beam electron diffraction.

Ultramicroscopy·2026
Same journal

Predictive drift compensation of multi-frame STEM via live scan modification.

Ultramicroscopy·2026
Same journal

Deep PACBED: Multitask analysis of PACBED images using deep neural networks.

Ultramicroscopy·2026
Same journal

Guided progressive reconstructive imaging: A new quantization-based framework for low-dose, high-throughput and real-time analytical ptychography.

Ultramicroscopy·2026
Same journal

Brightness optimization in a 200 keV DTEM source by geometry-driven aberration suppression.

Ultramicroscopy·2026
See all related articles

Related Experiment Video

Updated: May 24, 2026

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
10:25

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid

Published on: December 20, 2016

Minimizing tip-sample forces in jumping mode atomic force microscopy in liquid.

A Ortega-Esteban1, I Horcas, M Hernando-Pérez

  • 1Departamento de Física de la Materia Condensada, C-3, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.

Ultramicroscopy
|February 24, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces an improved atomic force microscopy jumping mode to accurately detect tip-sample contact, minimizing forces for better imaging of delicate biological samples like viruses.

More Related Videos

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid
08:58

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid

Published on: December 2, 2022

Related Experiment Videos

Last Updated: May 24, 2026

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
10:25

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid

Published on: December 20, 2016

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid
08:58

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid

Published on: December 2, 2022

Area of Science:

  • Biophysics
  • Materials Science
  • Nanotechnology

Background:

  • Minimizing tip-sample interaction forces is crucial for atomic force microscopy (AFM) performance, especially when imaging soft biological matter in liquid environments.
  • Cantilever dragging forces in liquid AFM hinder accurate tip-sample contact identification, leading to specimen damage.
  • Conventional jumping mode methods often fail to prevent deleterious interactions with delicate biological specimens.

Purpose of the Study:

  • To present an enhanced jumping mode procedure for atomic force microscopy (AFM).
  • To achieve high-accuracy detection of tip-sample contact during AFM scanning in liquid.
  • To minimize scanning forces during approach cycles for improved imaging of biological samples.

Main Methods:

  • Development of an improved jumping mode protocol for AFM.
  • Implementation of a procedure for accurate tip-sample contact detection.
  • Application of the method to image biological specimens in liquid.

Main Results:

  • The improved jumping mode procedure successfully detects tip-sample contact with high accuracy.
  • Scanning forces were minimized to approximately -100 pN during approach cycles.
  • High-resolution images of human adenovirus and T7 bacteriophage particles were obtained without uncontrolled modifications.

Conclusions:

  • The enhanced jumping mode offers a significant improvement for AFM imaging of soft biological matter in liquids.
  • Accurate tip-sample contact detection minimizes interaction forces, preserving specimen integrity.
  • This method enables reliable imaging of delicate biological structures like viruses and phages.