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Fig mosaic virus mRNAs show generation by cap-snatching.

Jeewan Jyot Walia1, Bryce W Falk

  • 1Department of Plant Pathology, University of California, Davis, CA 95616, USA.

Virology
|February 24, 2012
PubMed
Summary

Fig mosaic virus (FMV) generates messenger RNAs (mRNAs) with unique leader sequences. These mRNAs are capped using a cap-snatching mechanism, similar to other RNA viruses.

Area of Science:

  • Virology
  • Molecular Biology
  • Plant Pathology

Background:

  • Fig mosaic virus (FMV) belongs to the Emaravirus genus.
  • FMV possesses four negative-sense single-stranded RNA genomes, each encoding one protein in its viral complementary RNA (vcRNA).

Purpose of the Study:

  • To investigate the mRNA generation strategy of FMV.
  • To determine if FMV utilizes cap-snatching for mRNA synthesis.

Main Methods:

  • Analysis of 5' termini of FMV mRNAs from genome segments 2 and 3.
  • Utilizing the cap-binding protein eIF4E(K119A) to detect 5' capping.
  • Northern hybridization to analyze RNA structure and size.

Main Results:

  • FMV mRNAs for segments 2 and 3 possess short, heterogeneous 5' leader sequences.

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  • A population of FMV positive-sense RNAs are 5' capped, indicating cap-snatching.
  • Capped RNA3 segments are smaller than vcRNA3 and lack polyadenylation.

    • Conclusions:

    • FMV employs cap-snatching to produce 5' capped mRNAs.
    • This strategy is analogous to that used by other negative-sense multipartite ssRNA viruses.