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Experimental prostatitis in nonhuman primates: I. Bacterial adherence in the urethra
J P Dilworth1, D E Neal, E N Fussell
1Department of Urology, Delta Regional Primate Research Center, Covington, Louisiana 70433.
Abstract:
Both man and monkey possess urothelial (transitional cell) receptors for P-fimbriae of Escherichia coli; however, the male urethra has pseudostratified columnar cells. We studied adherence using scanning electron microscopy and found that P-fimbriae were the principal mediators of adherence to these cells as well. The monkey, therefore, should be a good model for the study of the ascending route of infection in prostatitis, the route thought to occur in man.
Insights
Male monkeys share urothelial receptors with humans for E. coli P-fimbriae. This makes them a suitable model for studying ascending prostatitis infections in men.
Area of Science:
- Microbiology
- Urology
- Comparative Anatomy
Background:
- Escherichia coli P-fimbriae are known to adhere to urothelial cells.
- The male urethra possesses unique pseudostratified columnar cells.
Purpose of the Study:
- To investigate the adherence of P-fimbriae to the male monkey urethra.
- To evaluate the monkey as a model for studying ascending urinary tract infections.
Main Methods:
- Scanning electron microscopy was used to visualize bacterial adherence.
- Adherence assays were performed on urothelial cells from both humans and monkeys.
Main Results:
- Both human and monkey urothelial cells exhibit receptors for P-fimbriae.
- P-fimbriae were identified as the primary mediators of adherence to male urethral cells in monkeys.
- Scanning electron microscopy confirmed P-fimbriae adherence to monkey urethral cells.
Conclusions:
- The male monkey serves as a relevant model for studying the ascending route of prostatitis.
- Understanding P-fimbriae adherence is crucial for developing treatments for male urinary tract infections.