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Related Experiment Video

Updated: May 24, 2026

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Published on: May 10, 2015

Cellular functions of the DUBs.

Michael J Clague¹, Judy M Coulson, Sylvie Urbé

¹Physiological Laboratory, Institute of Translational Medicine, University of Liverpool, Crown Street, L69 3BX Liverpool, UK. clague@liv.ac.uk

Journal of Cell Science

|February 24, 2012

Summary

This summary is machine-generated.

Deubiquitylating enzymes (DUBs) reverse ubiquitylation, a key cellular process. These enzymes regulate critical functions including protein stability, signaling pathways, and DNA repair.

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Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

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Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

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Published on: November 22, 2024

Area of Science:

Cellular Biology
Biochemistry
Molecular Biology

Background:

Ubiquitylation is a dynamic post-translational modification regulating cellular processes.
Deubiquitylating enzymes (DUBs) are crucial for reversing ubiquitylation.
The human genome encodes approximately 80 active DUBs with diverse specificities.

Purpose of the Study:

To discuss the broad cellular functions of DUBs.
To highlight the role of DUBs in key signaling pathways like Wnt and TGF-β.
To explore the nuclear functions of DUBs in transcription and DNA repair.

Main Methods:

Review and commentary on existing literature.
Analysis of DUBs' roles in cellular processes.
Discussion of DUBs' involvement in specific signaling pathways.

Main Results:

DUBs control protein stability, membrane traffic, and protein quality control.
Dynamic ubiquitylation regulated by DUBs is key in Wnt and TGF-β signaling.
Nuclear DUBs are essential for transcriptional activity and DNA repair.

Conclusions:

DUBs are critical regulators of diverse cellular functions.
Understanding DUBs is vital for comprehending complex cellular signaling and homeostasis.
DUBs play significant roles in both cytoplasmic and nuclear cellular processes.