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Related Concept Videos

Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
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Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
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Related Experiment Video

Updated: May 24, 2026

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
09:14

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Published on: January 14, 2016

Chromatin replication and epigenome maintenance.

Constance Alabert1, Anja Groth

  • 1Biotech Research and Innovation Centre, University of Copenhagen, Ole Maaløes Vej 5, DK2200 Copenhagen, Denmark.

Nature Reviews. Molecular Cell Biology
|February 24, 2012
PubMed
Summary
This summary is machine-generated.

Maintaining genome stability requires accurate DNA replication and chromatin organization during cell division. Disruptions to DNA synthesis can compromise both genome and epigenome integrity, impacting organism health.

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Last Updated: May 24, 2026

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Eukaryotic genome stability and function depend on chromatin structure.
  • Cell division necessitates accurate genome replication and chromatin landscape reproduction.
  • Chromatin and nuclear organization dictate DNA replication initiation sites and timing.

Purpose of the Study:

  • To explore the intricate relationship between DNA replication and chromatin organization.
  • To understand the mechanisms that maintain genome and epigenome integrity during cell division.
  • To investigate the consequences of perturbed DNA synthesis on cellular and organismal health.

Main Methods:

  • This study integrates knowledge from existing literature on DNA replication and chromatin dynamics.
  • It focuses on theoretical and mechanistic aspects rather than specific experimental procedures.
  • The analysis emphasizes the interplay between replication-coupled chromatin assembly and cell cycle epigenome maintenance.

Main Results:

  • DNA replication initiation is influenced by chromatin structure, while replication itself disrupts chromatin.
  • Specialized mechanisms exist for assembling new DNA into chromatin post-replication.
  • Epigenome maintenance is an ongoing process throughout the cell cycle.

Conclusions:

  • Perturbations in DNA synthesis challenge genome regulation and can lead to loss of genome and epigenome integrity.
  • Maintaining chromatin and epigenome integrity during replication is crucial for organismal well-being.
  • Further research into replication-coupled epigenetic maintenance is warranted.