Related Experiment Video
Updated: Jun 28, 2026

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
Published on: October 23, 2018
Hepatic protein and amino-acid metabolism in poultry
1Monsanto Company, Animal Sciences Division, Chesterfield, Missouri 63198.
Abstract:
Two subjects are discussed: first, the regulation of hepatic protein synthesis; and second, the intermediary metabolism of methionine, particularly with respect to the role of 2-hydroxy-4-(methylthio)-butanoic acid (HMB, Alimet). In the first section, the regulation of albumin synthesis is reviewed in terms of molecular events associated with the changes in albumin synthesis during fasting and refeeding. The effect of infection or of inflammatory stress on both albumin and total protein synthesis in the liver is also discussed. In the second part, research results are presented which indicate that HMB is a naturally occurring compound in methionine intermediary metabolism. The HMB synthesis by chick liver enzymes is demonstrated, and its role in normal avian methionine metabolism is discussed.
Related Concept Videos
Protein Digestion
Liver Physiology
Metabolic Regulation:
The liver is the central organ involved in regulating blood composition. It stabilizes blood glucose levels, maintaining them within the range of 70–110 mg/dL. When these levels drop, the liver breaks down glycogen reserves and releases glucose into the bloodstream. It can also...
Overview of Protein Metabolism
Amino acids play various roles in the body once they are absorbed into cells. They are restructured...
Inborn Errors of Metabolism
Amino Acid Catabolism
Amino Acid Biosynthetic Pathways

