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Updated: May 24, 2026

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation
Published on: March 20, 2018
Non random distribution of lesions induced by deoxyribonuclease I in human chromosomes
F Nuzzo1, A Casati, E Raimondi
1Instituto di Genetica Biochimica ed Evoluzionistica del C.N.R., Via Abbiategrasso 207, I-27100, Pavia, Italy.
Abstract:
We studied the action of deoxyribonuclease I on human lymphocytes in order to determine the localization of the DNAase induced aberrations. Our results indicate a non-random distribution of the lesions on chromosome regions which may reflect a differential pattern of sensitivity to the enzyme. Furthermore we observed a correspondence between the preferential DNAase induced breaks and fragile sites that are expressed in lymphocytes maintained in medium without folic acid. A possible interpretation of our findings is that the accessibility to DNAase and/or the efficiency of the repair systems depend on the chromatin structure that influences also the expression of some common fragile sites.
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