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Related Concept Videos

Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...

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Updated: May 24, 2026

Shear Assay Protocol for the Determination of Single-Cell Material Properties
08:19

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Published on: May 19, 2023

Shear sensitivity of insect cells.

J J Chalmers1

  • 1Department of Chemical Engineering, The Ohio State University, 140 West 19th Ave, 43210, Columbus, OH, U.S.A..

Cytotechnology
|February 24, 2012
PubMed
Summary
This summary is machine-generated.

Insect cells in bioreactors can be damaged by hydrodynamic forces, but this is preventable. Using specific surface-active compounds like Pluronic F-68 prevents cell attachment to interfaces, protecting insect cells from damage.

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Area of Science:

  • Biotechnology
  • Cell Culture Engineering

Background:

  • Insect cells are susceptible to damage in bioreactors due to hydrodynamic forces.
  • Cell attachment to gas-liquid interfaces is a primary mechanism of damage.

Purpose of the Study:

  • To identify and explain the mechanisms of insect cell damage in bioreactors.
  • To present methods for preventing cell damage in bioreactor environments.

Main Methods:

  • Investigated hydrodynamic forces and interface phenomena affecting insect cells.
  • Evaluated the efficacy of surface-active compounds in preventing cell attachment.

Main Results:

  • Cell attachment to gas-liquid interfaces leads to physical removal or death.
  • Surface-active compounds effectively prevent cell adhesion to interfaces.

Conclusions:

  • Insect cell damage in bioreactors is largely preventable.
  • Pluronic F-68 and Methocel E-50 are effective agents for protecting insect cells by preventing interface attachment.