Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Bioreactor Controls-I01:28

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
Designing Growth Media for Bioreactors01:30

Designing Growth Media for Bioreactors

Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Current good manufacturing practice in plant automation of biological production processes.

Cytotechnology·2012
Same author

Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers.

Cytotechnology·2012
Same author

Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers.

Cytotechnology·2012
Same author

Animal cell culture: Towards the 21st century.

Cytotechnology·2012
Same author

Expression of phosphofructokinase in Neisseria meningitidis.

Microbiology (Reading, England)·2009
Same author

A practical approach for exploration and modeling of the design space of a bacterial vaccine cultivation process.

Biotechnology and bioengineering·2009

Related Experiment Video

Updated: May 24, 2026

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells
08:15

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

Published on: September 28, 2018

Batch control system vaccines: BCSV : A new man machine interface for bioreactors.

G Wieten1, R C Dorresteijn, M C Philippi

  • 1RIVM, P.O. Box 1, 3720 BA, Bilthoven, The Netherlands.

Cytotechnology
|February 24, 2012
PubMed
Summary

A new Batch Control System for Vaccines (BCSV) enhances bioreactor cultivations using SP-88 standards. This system optimizes processes and ensures Good Manufacturing Practice (GMP) compliance through flexible recipe management and operator interaction.

More Related Videos

Large-Scale Cell Production Based on GMP-Grade Dissolvable Porous Microcarriers
09:44

Large-Scale Cell Production Based on GMP-Grade Dissolvable Porous Microcarriers

Published on: July 7, 2023

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
09:28

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

Published on: May 18, 2020

Related Experiment Videos

Last Updated: May 24, 2026

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells
08:15

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

Published on: September 28, 2018

Large-Scale Cell Production Based on GMP-Grade Dissolvable Porous Microcarriers
09:44

Large-Scale Cell Production Based on GMP-Grade Dissolvable Porous Microcarriers

Published on: July 7, 2023

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
09:28

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

Published on: May 18, 2020

Area of Science:

  • Biotechnology and Pharmaceutical Manufacturing
  • Automation and Control Systems

Background:

  • Bioreactor cultivation requires precise control for process optimization and regulatory compliance.
  • Existing systems may lack standardization and flexibility across different scales.
  • The ISA SP-88 standard provides a framework for batch control systems.

Purpose of the Study:

  • To develop a standardized Batch Control System for Vaccines (BCSV) for bioreactor cultivations.
  • To enhance process optimization and ensure compliance with Good Manufacturing Practice (GMP) regulations.
  • To create a flexible and user-friendly Man Machine Interface (MMI).

Main Methods:

  • Development of the BCSV according to the ISA SP-88 standard for batch control.
  • Implementation of a recipe-based approach for organizing monitoring and control requirements.
  • Designing an independent interface for hardware flexibility and consistent user experience.
  • Integrating interactive operator communication during recipe execution.

Main Results:

  • The BCSV facilitates knowledge and experience transfer across production, pilot, and R&D scales.
  • Hardware independence ensures a uniform "look and feel" across diverse equipment.
  • In-house recipe management provides greater control over applications.
  • Interactive communication enhances operator engagement during batch execution.

Conclusions:

  • The BCSV system successfully integrates process optimization and GMP compliance in vaccine manufacturing.
  • The system's design promotes scalability and adaptability in bioreactor operations.
  • A comprehensive QA strategy ensures system integrity throughout its lifecycle.