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An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates
Published on: January 7, 2019
Agitation and aeration effects in suspension mammalian cell cultures
C P Marquis1, K S Low, J P Barford
1Dept. of Infectious Diseases, The University of Sydney, 2006, NSW, Australia.
Abstract:
Three different hybridoma cell lines, grown in serum-free media with different levels of Pluronic F-68, were subjected to a shear force of 0.6 N m(-2). Some protective effect due to the polymer was found, indicating it to be a potentially useful adjuvant in serum-free media. Other observations of liquid and gas effects at the reactor level have been included here. A discussion of the difference between suspension and microcarrier cultures, in relation to hydrodynamic effects, is included.
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