Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 24, 2026

Rescue of Recombinant Newcastle Disease Virus from cDNA

Rescue of Recombinant Newcastle Disease Virus from cDNA

Published on: October 11, 2013

New applications of alphavirus-based expression vectors.

M Boorsma¹, D Koller, W A Renner

¹Cytos Biotechnology AG, Wagistrasse 21, CH-8952, Zurich-Schlieren, Switzerland.

|February 24, 2012

Summary

Alphavirus vectors offer high-level protein production for gene therapy and vaccine development. New non-cytopathic and inducible vectors enhance bioprocess strategies and gene discovery.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Changes in plasma glutamate in hemodialysis-related-headache patients: A pilot study.

Neurologia·2026

Same author

Refining the hospitalization rate: A mixed methods approach to differentiate primary COVID-19 from incidental cases.

Infection prevention in practice·2024

Same author

[Relevance of health geographic research for dermatology].

Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete·2021

Same author

LIGHT/LTβR signaling regulates self-renewal and differentiation of hematopoietic and leukemia stem cells.

Nature communications·2021

Same author

Analysis of soluble or titanium dioxide derived titanium levels in human whole blood: consensus from an inter-laboratory comparison.

The Analyst·2018

Same author

Allergens displayed on virus-like particles are highly immunogenic but fail to activate human mast cells.

Allergy·2017

Area of Science:

Molecular Biology
Virology
Biotechnology

Background:

Alphaviruses, such as Sindbis virus and Semliki Forest virus, are positive-stranded RNA viruses known for high-titer replication.
Viral vectors derived from these alphaviruses are established tools for producing recombinant proteins.
Applications have expanded into vaccine production, gene therapy, and cellular function analysis.

Purpose of the Study:

To discuss the development of advanced alphavirus expression vectors.
To highlight the utility of non-cytopathic and inducible vectors for bioprocess development.
To introduce a Sindbis-based system for rapid functional gene identification.

Main Methods:

Development of non-cytopathic and inducible alphavirus expression vectors.

More Related Videos

Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors

Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors

Published on: November 24, 2010

Related Experiment Videos

Last Updated: May 24, 2026

Rescue of Recombinant Newcastle Disease Virus from cDNA

Rescue of Recombinant Newcastle Disease Virus from cDNA

Published on: October 11, 2013

Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors

Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors

Published on: November 24, 2010

Construction of a Sindbis virus-based expression cloning system.

Application of these systems for recombinant protein production and gene discovery.

Main Results:

Established non-cytopathic and inducible alphavirus vectors suitable for bioprocessing.
Demonstrated high-level production of recombinant proteins using these vectors.
Developed a cloning system enabling rapid identification of genes encoding proteins with specific functions.

Conclusions:

Alphavirus vectors are versatile tools for biotechnology, offering efficient protein production.
Novel vector designs enhance applicability in bioprocess development and gene therapy.
The Sindbis-based system accelerates the discovery of functional genes.