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Individuation of subcellular targets of antitumor agents by ultrastructural methods

G Arancia1

  • 1Department of Ultrastructures, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy.

Cytotechnology
|February 24, 2012
PubMed
Abstract

No abstract available in PubMed .

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Subcellular Fractionation01:32

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The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
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