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Related Experiment Video

Updated: May 24, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Single cell assay with an automated capillary microinjection system.

R Pepperkok1, C Schneider, L Philipson

  • 1European Molecular Biology Laboratory, Heidelberg, Germany.

Cytotechnology
|February 24, 2012
PubMed
Summary
This summary is machine-generated.

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This study introduces an automated microinjection system for high-throughput cell injections. The system achieves over 90% success rates and improves experimental accuracy for applications like mRNA research.

Area of Science:

  • Cell Biology
  • Biotechnology
  • Microfluidics

Background:

  • Manual microinjection is time-consuming and lacks reproducibility.
  • Automated systems are needed for high-throughput cell-based assays.
  • Accurate delivery of molecules into cells is crucial for functional studies.

Purpose of the Study:

  • To describe an automated capillary microinjection system.
  • To evaluate its applications and advantages.
  • To demonstrate its utility in molecular and cell biology research.

Main Methods:

  • Computer-controlled positioning of cells and capillaries for automated microinjection.
  • Development of methods for glass capillary preparation and handling.
  • Use of FITC-Dextran as a coinjection marker to assess cell viability and retrieval.

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Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
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Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Related Experiment Videos

Last Updated: May 24, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
07:55

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

  • Application in functional screening of cDNA libraries and mRNA molecules.

    • Main Results:

    • Achieved approximately 1500 injections per hour with high reproducibility.
    • Reported successful injection rates of over 90% for cytoplasmic and 85% for nuclear injections.
    • Demonstrated 99% cell retrieval 48 hours post-injection using a fluorescent marker.
    • Confirmed the existence of mRNA inhibiting cell proliferation.

    Conclusions:

    • The automated system significantly enhances efficiency and accuracy in cell microinjection.
    • It enables high-throughput functional screening of genetic material.
    • Improved cell handling and reduced capillary clogging contribute to reliable experimental outcomes.