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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

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Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
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Label-free quantification using MALDI mass spectrometry: considerations and perspectives.

Amelie S Benk1, Christoph Roesli

  • 1Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH), Heidelberg, Germany.

Analytical and Bioanalytical Chemistry
|February 24, 2012
PubMed
Summary

Understanding protein changes in disease requires advanced techniques. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) combined with ultra-high-performance liquid chromatography (UHPLC) shows promise, but software limitations hinder its full potential for protein quantification.

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Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
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Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)
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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Proteomics

Background:

  • Knowledge of protein abundances in healthy and diseased tissues is vital for biological understanding and developing diagnostics/therapeutics.
  • Mass spectrometry-based label-free protein quantification offers insights into physiological changes in perturbed systems.

Purpose of the Study:

  • To review bioinformatic data-processing tools for label-free quantification.
  • To elaborate on the technical benefits of combining ultra-high-performance liquid chromatography (UHPLC) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS).
  • To outline the potential of state-of-the-art instruments using unpublished results from complex biological samples.

Main Methods:

  • Focus on instrumental setups utilizing a MALDI ion source for protein quantification.
  • Review of current bioinformatic data-processing tools for label-free quantification.
  • Analysis of twenty-four complex biological samples using LC-MALDI MS.

Main Results:

  • Demonstration of the technical benefits of combining UHPLC and MALDI-MS.
  • Presentation of unpublished results from twenty-four complex biological samples.

Conclusions:

  • The capabilities of LC-MALDI MS systems for protein quantification are currently underutilized.
  • A lack of suitable software tools is a significant barrier to fully exploiting LC-MALDI MS systems.