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Polystyrene substratum for bulk culture of anchorage dependent cells

A Kadouri1, D Sher, N G Maroudas

  • 1Laboratory of Applied Enzymology, Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel.

Cytotechnology
|February 24, 2012
PubMed

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Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media