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Related Concept Videos

Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...
Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.

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Related Experiment Video

Updated: May 24, 2026

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
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Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

The capsicum transcriptome DB: a "hot" tool for genomic research.

Elsa Góngora-Castillo, Rubén Fajardo-Jaime, Araceli Fernández-Cortes

    Bioinformation
    |February 24, 2012
    PubMed
    Summary

    Researchers developed a genomic resource for chili pepper (Capsicum annuum) by sequencing expressed sequence tags (ESTs) from multiple organs. This resource provides novel gene sequences and a searchable database to advance Capsicum annuum research.

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    High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
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    High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

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    Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
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    Published on: December 22, 2017

    High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
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    High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

    Published on: March 5, 2022

    Area of Science:

    • Genomics
    • Plant Science
    • Bioinformatics

    Background:

    • Chili pepper (Capsicum annuum) is a vital crop with limited public genomic data.
    • Genomic resources are crucial for understanding and improving crop traits.

    Purpose of the Study:

    • To create a comprehensive genomic resource for Capsicum annuum.
    • To facilitate future research and genetic studies in chili peppers.

    Main Methods:

    • Sequencing of expressed sequence tags (ESTs) from five C. annuum organs using Sanger sequencing.
    • Deep sequencing of leaf transcriptomes using GS-pyrosequencing.
    • Hybrid assembly of raw reads to generate high-quality contigs.

    Main Results:

    • A hybrid assembly yielded 32,314 high-quality contigs from over 1.3 million reads.
    • 75.5% of contigs showed significant similarity to existing database entries.
    • 23% of sequences were novel for C. annuum, expanding available genomic information.

    Conclusions:

    • The developed genomic resource, including a searchable database, significantly enhances the available sequence data for Capsicum annuum.
    • This resource will accelerate research in chili pepper genetics, breeding, and functional genomics.